plasmid fuw dcas9 dnmt3a (Addgene inc)
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plasmid fuw dcas9 dnmt3a
Plasmid Fuw Dcas9 Dnmt3a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fuw+dcas9+dnmt3a/Fuw-dCas9-Dnmt3a+(Plasmid+%2384476)/pm41013732-72-0-8
Average 93 stars, based on 18 article reviews
Plasmid Fuw Dcas9 Dnmt3a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fuw+dcas9+dnmt3a/Fuw-dCas9-Dnmt3a+(Plasmid+%2384476)/pm41013732-72-0-8
Average 93 stars, based on 18 article reviews
plasmid fuw dcas9 dnmt3a - by Bioz Stars,
2026-09
93/100 stars
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Plasmid Preparation:Article Title: Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells. Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, USA) and the cells were cultured at 37 °C in a humidified incubator with 5% CO2. .. The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1A) of Article Title: Abnormal hypo-methylation of CAP1 (adenylate cyclase associated protein 1) gene enhances biological characteristics of lung adenocarcinoma cells Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scienti c, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scienti c, USA) and the cells were cultured at 37°C in a humidi ed incubator with 5% CO2. .. Plasmid design and construction The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1a) of Article Title: Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, USA) and the cells were cultured at 37 °C in a humidified incubator with 5% CO 2 . .. The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1A) of Amplification:Article Title: Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells. Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, USA) and the cells were cultured at 37 °C in a humidified incubator with 5% CO2. .. The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1A) of Article Title: Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, USA) and the cells were cultured at 37 °C in a humidified incubator with 5% CO 2 . .. The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1A) of Polymerase Chain Reaction:Article Title: Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells. Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, USA) and the cells were cultured at 37 °C in a humidified incubator with 5% CO2. .. The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1A) of Article Title: Abnormal hypo-methylation of CAP1 (adenylate cyclase associated protein 1) gene enhances biological characteristics of lung adenocarcinoma cells Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scienti c, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scienti c, USA) and the cells were cultured at 37°C in a humidi ed incubator with 5% CO2. .. Plasmid design and construction The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1a) of Article Title: Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells Article Snippet: All media were supplemented with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, USA) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, USA) and the cells were cultured at 37 °C in a humidified incubator with 5% CO 2 . .. The UBC-dCas9-Dnmt3a domain (red frame segment) (Fig. S1A) of Construct:Article Title: Locus-Specific DNA Methylation Editing in Melanoma Cell Lines Using a CRISPR-Based System Article Snippet: .. The sequences for these effector constructs were derived from the following commercially available plasmids, respectively: Derivative Assay:Article Title: Locus-Specific DNA Methylation Editing in Melanoma Cell Lines Using a CRISPR-Based System Article Snippet: .. The sequences for these effector constructs were derived from the following commercially available plasmids, respectively: Cell Culture:Article Title: CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer's disease. Article Snippet: Cell lines were authenticated by STR (Short Tandem Repeat) analysis (Kogene Biotech, Seoul, Korea), and mycoplasma was tested using a MycoSensor PCR assay kit (Agilent, Santa Clara, CA). .. For lentivirus production, HEK293T cells were passaged and cultured at 80% confluency and transfected with lentiviral vectors (psPAX2, pMD2.G, and pLenti-sgRNA [Addgene, Cambridge, MA, #71409] or Article Title: CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer’s disease Article Snippet: Cell lines were authenticated by STR (Short Tandem Repeat) analysis (Kogene Biotech, Seoul, Korea), and mycoplasma was tested using a MycoSensor PCR assay kit (Agilent, Santa Clara, CA). .. For lentivirus production, HEK293T cells were passaged and cultured at 80% confluency and transfected with lentiviral vectors (psPAX2, pMD2.G, and pLenti-sgRNA [Addgene, Cambridge, MA, #71409] or Transfection:Article Title: CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer's disease. Article Snippet: Cell lines were authenticated by STR (Short Tandem Repeat) analysis (Kogene Biotech, Seoul, Korea), and mycoplasma was tested using a MycoSensor PCR assay kit (Agilent, Santa Clara, CA). .. For lentivirus production, HEK293T cells were passaged and cultured at 80% confluency and transfected with lentiviral vectors (psPAX2, pMD2.G, and pLenti-sgRNA [Addgene, Cambridge, MA, #71409] or Article Title: CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer’s disease Article Snippet: Cell lines were authenticated by STR (Short Tandem Repeat) analysis (Kogene Biotech, Seoul, Korea), and mycoplasma was tested using a MycoSensor PCR assay kit (Agilent, Santa Clara, CA). .. For lentivirus production, HEK293T cells were passaged and cultured at 80% confluency and transfected with lentiviral vectors (psPAX2, pMD2.G, and pLenti-sgRNA [Addgene, Cambridge, MA, #71409] or |